The Effect of Bay 11-7082 Inhibits Rat BMSCs to Hepatocyte-Like Cells via NF-κB Signaling in Vitro

Author(s): Xiaoping Liu, Tongxi Yang, Yi Wang, Shasha Jiang, Zhongjie Yu

Bone marrow stem cells (BMSCs) could be induced into hepatocytes by different cytokines, which has a great potential in tissue engineering. However, the differentiation was not so efficient mainly due to the less understanding of molecular mechanism. Our objective was to investigate the effect of BAY 11-7082 on the differentiation of BMSCs into hepatocyte-like cells. We observed the majority of differentiated cells were spherical or ovate in shape, indocyanine green (ICG) positive, α-1-antitrypsin (AAT) expression increased and NF-кB transferred into nuclear. Whereas the BMSCs additionally added with BAY 11-7082, which were showed fusiform or polygonal morphologies unchanged in shape, ICG uptake and AAT expression reduced, NF-кB scattered in the cytoplasm. These data suggest the activation of BAY 11-7082 may inhibit the differentiation of BMSCs into hepatocyte-like cells via NF-кB signaling.

BMSCs, Hepatocyte, Differentiation, NF-кB, Indocyanine green, BAY 11-7082,

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