Effect of Stereotactic Hematoma Aspiration on the NSE Expression after Experimental Intracerebral Hemorrhage

Author(s): Yanan Wang, Zhaoxing Li, Chengjie Sun, Xiaoying Wang, Kai Luo, Hongyan Zhang, Ning Gu, Dehui Zhu

Objective: To observe the effect of stereotactic hematoma aspiration on the expression of neuron-specific enolase (NSE) in perihematomal tissue after intracerebral hemorrhage. Methods: The model of intracerebral hemorrhage were established by using inracerebral stereotaxic injection of type IV collagenase method in male adult Wistar rats and were randomly divided into sham operation group, intracerebral hemorrhage group, and hemorrhage aspiration group. The neurological impairment in rats were evaluated by means of Bederson four grade. The morphology of nerve cells in cortex were observed by the hematoxylin-eosin staining. The expression of NSE in cortex was detected respectively by the immunohistochemistry and Western Blot. Results: The expression of NSE in the peripheral region of hematoma in hematoma aspiration group and intracerebral hemorrhge group was significantly higher than that in sham operation group each time point after intracerebral hemorrhage (P<0.05). The expression of NSE in the peripheral region of hematoma in hematoma aspiration group was slightly higher than that of intracerebral hemorrhage ground one day after intracerebral hemorrhage (P<0.05), but there was no significantly difference. Compared with intracerebra hemorrhage group, the expression of NSE in the peripheral region of hematoma in hemorrhage aspiration group was significantly decreased on 3rd and 5th day after intracerebral hemorrhage. Compared with hemorrhage aspiration group, the numbers of positive cells were significantly increased and the damage of the neurons in cortex was worsened than that in intracerebra hemorrhage group. Conclusion: The expression of NSE in perihematomal tissue after intracerebral hemorrhage was increased obviously after intracerebral hemorrhage. However, early hematoma aspiration could weaken the expression of NSE, reduce the number of neuronal degeneration cells, and improved neurobehavioral function significantly after intracerebral hemorrhage.

NSE, Intracerebral Hemorrhage, Stereotactic Hematoma Aspiration, Rat

1. Wold F, Ballou CE. Studies on the enzyme enolase. I. Equilibrium studies[J]. J Biol Chem, 1957, 227(1):301-312.
2. Tolan NV, Vidal-Folch N, Algeciras-Schimnich A, et al. Individualized correction of neuron-specific enolase (NSE) measurement in hemolyzed serum samples[J]. Clin Chim Acta, 2013, 424(2013):216-221. http://dx.doi.org/10.1016/j.cca.2013.06.009
3. Bederson JB, Pitts LH, Tsuji M, et al. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination[J]. Stroke, 1986, 17(3):472-476. http://dx.doi.org/10.1161/01.str.17.3.472
4. Peeling J, Yan HJ, Chen SG, et al. Protective effects of free radical inhibitors in intracerebral hemorrhage in rat[J]. Brain Res, 1998, 795(1-2):63-70. http://dx.doi.org/10.1016/s0006-8993(98)00253-4
5. Kawai N, Nakamura T, Nagao S. Early hemostatic therapy using recombinant factor VIIa in a collagenase-induced intracerebral hemorrhage model in rats[J]. Acta Neurochir Suppl, 2006, 96:212-217. http://dx.doi.org/10.1007/3-211-30714-1_46
6. Zhang HB, Zhang SM. Rat model of cerebral hemordlage. Foreign Med Sci (Cereb Dis), 2002, 10(6):469-472.
7. Wu YC, Zhao YB, Lu CZ, et al. Correlation between serum level of neuron-specific enolase and long-term functional outcome after acute cerebral infarction: prospective study. Hong Kong Med J, 2004, 10(4):251-254.
8. Soh M, Dunlevy J R, Garrett S H, et al. Increased neuron specific enolase expression by urothelial cells exposed to or malignantly transformed by exposure to Cd(2)(+) or As(3)(+)[J]. Toxicol Lett, 2012, 212(1):66-74. http://dx.doi.org/10.1016/j.toxlet.2012.05.003
9. Chiaretti A, Barone G, Riccardi R, et al. NGF, DCX, and NSE upregulation correlates with severity and outcome of head trauma in children. Neurology, 2009, 72(7):609-616. http://dx.doi.org/10.1212/01.wnl.0000342462.51073.06
10. Hattori T, Takei N, Mizuno Y, et al. Neurotrophic and neuroprotective effects of neuron-specific enolase on cultured neurons from embryonic rat brain. Neurosci Res, 1995, 21(3):191-198. http://dx.doi.org/10.1016/0168-0102(94)00849-b
11. Hatfield RH, Mckernan RM. CSF neuron-specific enolase as a quantitative marker of neuronal damage in a rat stroke model. Brain Res, 1992, 577(2):249-252. http://dx.doi.org/10.1016/0006-8993(92)90280-m
12. Garcia-Alix A, Cabanas F, Pellicer A, et al. Neuron-specific enolase and myelin basic protein: relationship of cerebrospinal fluid concentrations to the neurologic condition of asphyxiated full-term infants. Pediatrics, 1994, 93(2):234-240.
13. Huang GB, Wei SZ, Yao LT, et al. Correlation between serum NSE, Protein S100β and cognitive dysfunction of cerebral hemorrhagic patients. Journal of Brain and Nervous Diseases, 2012, 20(0)251-255.
14. Yang XP, Li QF, Yang RL. Dynamic changes and significance of serum neuron specific enolase during acute period in patients with intracerebral hemorrhage. Chinese Journal of Practical Nervous Diseases, 2008, 11(09):37-39.
15. Lin J, Wang HO, Lin FC, et al. The significance and change of neuron-specific enolase in acute cerebral hemorrhage of rabbit. Chinese Journal of Practical Nervous Diseases, 2006, 9(01):41-43.
16. Singh HV, Pandey A, Shrivastava AK, et al. Prognostic value of neuron specific enolase and IL-10 in isReferenceschemic stroke and its correlation with degree of neurological deficit. Clin Chim Acta, 2013, 419(2013):136-138. http://dx.doi.org/10.1016/j.cca.2013.02.014
17. Herrmann M, Ebert AD, Galazky I, et al. Neurobehavioral outcome prediction after cardiac surgery: role of neurobiochemical markers of damage to neuronal and glial brain tissue. Stroke, 2000, 31(3):645-650. http://dx.doi.org/10.1161/01.str.31.3.645