Antigerminative Effects of Ethanolic Crude Extracts from Devil Tree (Alstonia scholaris) on Seed Germination and Seedling Growth against Selected Plants

Author(s): Sawatdikarn Sanit

The study of ethanolic crude extracts from the leaves of devil tree (Alstonia scholaris) were extracted by 95% ethanol and used at six concentrations (0 0.5 1.0 1.5 2.0 and 2.5 g/L). The six concentrations of devil tree crude extracts were tested on the inhibitory on seed germination and seedling growth of six tested crops (mimosa (Mimosa pigra), chinese kale (Brassica oleracea), amaranthus (Amaranthus viridis), barnyard grass (Echinochloa crus-galli), weedy rice (Oryza sativa) and waxy corn (Zea mays). Inhibitory of five characteristics (seed germination, shoot length, root length, seedling fresh weight and seedling dry weight) were recorded. The results showed that all crude extracts of devil tree (0.5 1.0 1.5 2.0 and 2.5 g/L concentrations) inhibited seed germination and seedling growth of six tested plants for 9-100% inhibition. whereas, the devil tree crude extracts at 2.5 g/L concentration showed the highest inhibition of seed germination and seedling growth in term of shoot length, root length, seedling fresh weight and seedling dry weight characteristics at 100% inhibition against three tested plants (mimosa, chinese kale as well as amaranthus). A raise of concentration of devil tree crude extracts showed increased inhibition of seed germination and seedling growth in six tested plants. The result is showed the antigerminative effects of devil tree (A. scholaris) as well as suggested that the utilization of the ethanolic crude extracts from the leaves of devil tree (A. scholaris) so as to weed management.

Ethanolic Crude Extracts, Devil Tree, Seed Germination Inhibition, Seedling Growth Inhibition, Antigerminative Effects

1. Amb, M. K. and A. S. Ahluwalia. 2016. Allelopathic: potential role to achieve new milestones in rice cultivation. Rice Science. 23:165-183.
2. Ameena, M., A. L. Geethakumari and S. George. 2015. Allelopathic effects of root exudates of purple nut sedge (Cyperus rotundus L.) on growth of field crops. Journal Crop and Weed. 11:142-145.
3. AOSA. 2002. Seed Vigor Testing Handbook. Association of Official Seed Analysts. No.32.
4. Arulmozhi, S., M. P. Mazumder., P. Ahok and L. S. Narayanan. 2007. Pharmacological activities of Alstonia scholaris Linn (Apocynaceae)- A review. PHCOG. Rev. 1:163-170.
5. Ayeni, M. J. 2017. Effect of aqueous extracts of the leaves of Senna siamea Lam. and Pinus carribaea (Jacq.) ex. Walp. on the germination and seedling growth of Euphorbia heterophylla L. World Scientific News. 87:12-23.
6. Azhar, W., Jabeen, K., Iqbal, S. 2016. Appraisal of Alstonia scholaris antifungal activity against fusarium wilt. J. Agric. Res. 54, 221-231.
7. Chengxu, W., Z. Mingxing., C. Xuhui and Q. Bo. 2011. Review on allelopathic of exotic invasive plants. Procedia Engineering. 18:240-246.
8. Dukpa, R., A. Tiwari and D. Kapoor. 2020. Biological management of alleliphathic plant Parthenium sp. Open Agriculture. 5:252-261.
9. El-Askary, H. I., M. M. El-Olemy., M. M, Salama., A. A. Sleem and M. H. Amer. 2012. Bioguided isolation of pentacyclic triterpenes from the leaves of Alstonia scholaris (L.) R. Br. Growing in Egypt. Natural Product Research. 26:1755-1758.
10. El-Shora., Y. A. El-Amier and M. H. Awad. 2016. Antimicrobial activity and allelopathic activity of Zygophyllum coccineum L. on Chenopodium album L.. British Journal of Applied Science & Technology. 15:1-10.
11. Hameed, M., S. Fatima., S. M. R. Shah., F. Ahmad., M. Ashraf., Maryam., M. S. A. Ahmad., I. Ahmad and U. Iqbal. 2020. Ultrastructural response of wheat (Triticum aesivum L.) lines to potential allelopathy of Alstonia scholaris. Turkish Journal of Botany. 44:509-525.
12. Iqbal, J. and Z. A. Cheema. 2008. Purple nutsedge (Cyperus rotundus L.) management with combined application of sograab and S-metolachlor. Pak. J. Bot. 40:2383-2391.
13. ISTA. 2003. ISTA Handbook on Seedling Evaluation, 3 rd Edition. International Rules for Seed Testing 2003.
14. Jabran, K., G. Mahajan., V. Sardana and B. S. Chauhan. 2015. Allelopathy for weed control in agricultural systems. Crop Protection. 72: 57-65.
15. Javaid, A., S. Shafique., R. Bajwa and S. Shafique. 2010. Parthenium management through aqueus extracts of Alstonia scholaris. Pak. J. Bot. 42:3651-3657.
16. Julio, A., W. C. Tandoc., H. D. Tipace., Y. F. Vendivil., Z. Yanesa., M. V. R. Tare., E. J. Lactoaen and K. J. Clemente. 2019. Allelopathic effect of Lantana camara and Chromolaena odorata leaf extracts on plant germination. Asian J. Agric. & Biol. 9:190-196.
17. Naseem, M., M. Aslam., M. Ansar and M. Azhar. 2009. Allelopathic effects of sunflower water extract on weed control and wheat productivity. Pak. J. Weed Sci. Res. 15:107-116.
18. Nekonam, M. S., J. Razmjoo., H. Kraimmojeni., B. Sharifnabi., H. Amini and F. Bahrami. 2014. Assessment of some medicinal plants for the allelopathic potential against redroot pigweed (Amaranthus retroflexus). Journal of Plant Protection Research. 54: 90-95.
19. Rusdy, M., M. Riadi., A. M. Sari and A. Normal. 2015. Comparative Allelopathic effect of Imperata cylindrical and Chromalaena odorata on germination and seedling growth of Centronema pubescens. International Journal of Scientific and Research publicaations. 5: 1-5.
20. Sawatdikarn, S. 2010. Effects of some crude extracts on inhibition of growth in purple nutsedge (Cyperus rotundus L.), p. 1-6. In Proceeding of The 36 th Congress on Science and Technology of Thailand.
21. Sawatdikarn, S. 2017. In vitro allelopathic effects of ethanolic crude extracts from six members in Piper genus on seed germination and seedling growth in Black gram (Vigna mungo Hepper.). p. 89-98. In Proceeding of the International Scientific Conference on Engineering and Applied Sciences.
22. Sawatdikarn, S. 2020. In vitro allelopathic effects of Eehanolic crude extracts from six medicinal herbs on seed germination and seedling growth in black gram (Vigna mungo Hepper.). p. 178-190. In Proceeding of the 7 th Annual Conference on Engineering and Information Technology.
23. Sawatdikarn, S., S. Teanthong., N. Pradabpet., W. Rungprom and S. Siripornvisal. 2011. Crude extraction from bark and leaves of devil tree (Alstonia scholaris) and effect of crude extracts on seed germination and growth of some crops. p. 596-606. In Proceeding of 49 th Kasetsart University Annual Conference.
24. Shafique, S., A. Javaid., R. Bajwa and S. Shafique. 2007. Effect of aqueous leaf extracts of allelopathic trees on germination and seed-borne mycoflora of wheat. Pak. J. Bot. 39:2619-2624.
25. Shehata, H. F. 2014. Allelopathic potential of Portulaca oleracea L. seed extracts on germination and seedling growth of Cichorium endivia L., Lactuca sativa L., Echinochloa crus-galli L. and Brassica tournefortii Gouan. Journal of Experimental Biology and Agricultural Sciences. 2:388-396.
26. Wang, C., H. Chen., Y. Li., J. Weng., Y. Jhan., S. Lin and C. Chou. 2014. The role of pentacyclic triterpenoids in the allelopathic effects of Alstonia scholaris. J. Chem. Ecol. 40:90-98.
27. Wang, F., F. Ren and J. Liu. 2009. Alstonic acid A and B, unusual 2,3-secofernane triterpenoids from Alstonia scholaris. Phytochemistry. 70:650-654.
28. Zhou, H., H. P. He., S. D. Luo., Y. H. Wang., X. W. Yang., Y. T. Di and X. J. Hao. 2005. Three new indole alkaloids from the leaves of Alstonia scholaris. Helv. Chim. Acta. 88:2508-2512.