Qualitative and Quantitative Screening of Actinomycetes from Farmland and Rhizospheric Soil

Author(s): Oguoma O.I., Oguoma L.M.O., Nwokediuko I.I., Emeka-Nwabunnia I., Ezejiofor CC

Farmland soil and rhizospheric soil were screened for the presence of antibiotic producing Actinomycetes. Using culture based techniques. Isolates were isolated suaing two media: Starch Casein Agar medium and Actinomycetes Isolation Agar (AIA) medium. Isolates were characterized morphologically and biochemically and identified to be in the genus Actinomycetes. Ten isolates were isolated with varied qualitative and quantitative spectrum of activity against Multidrug resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Candida albicans. The quantitative antimicrobial assay recorded a zone of inhibition of 0-26mm diameter of zone of inhibition. Results show that the soil samples are important sources of antibiotics for the fight of Multidrug resistant isolates.

Actinomycetes, Antibiotics, Multidrug Resistance, Rhizopheric Soil

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