Isatin Inhibits SH-SY5Y Neuroblastoma Cell Invasion and Metastasis through LSD1 Activity Inhibition

Author(s): Shaobo Cong, Lin Hou, Haoyue Luo, Yanan Hua, Xue Li, Fangling Wang, Li Zhang, Zheng Zhang, Ning Li

Isatin has received much attention in recent years due to its anti-cancer properties[1], which offer important medical benefits. Isatin is an endogenous oxidized indole with a wide spectrum of behavioral and metabolic effects[2] and is commonly found in mammalian tissues and fluids[3]. It has many possible uses on the biomedical field[4] [5]and has also been investigated as a potential anti-cancer drug. However, its effects on neuroblastoma (NB) cells is still a mystery. This research aimed to elucidate the effects of Isatin on neuroblastoma cells metastasis and invasion and the underlying mechanism. Neuroblastoma cells viability was tested by CCK8. NB cells invasion and migration ability were tested by transwell and wound healing experiment. The mRNA relative expression of related molecules are detected by Rt-PCR and q-PCR. The protein relative expression of related molecules are detected by Simple western blotting. Our results demonstrated that isatin could inhibit neuroblastoma cell proliferation, invasion, and migration in a dose-dependent manner. Moreover, isatin increases the expression level of H3K4m1, PTEN. All results support the potential anti-metastatic effect of isatin in neuroblastoma cells.

Isatin, Neuroblastoma, PTEN, LSD1, Invasion

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