Therapeutic Effect of Down-Regulation of MTH1 Gene Expression on HepG2 Hepatocellular Carcinoma in Nude Mice

Author(s): Fuping Guan, Zhao Na, Yinlin Ge

The aim of this study was to establish an animal model of HepG2 liver cancer in nude mice, and to inhibit the expression of MTH1 gene in tumor cells by siRNA interference. To study the therapeutic effect of down-regulation of MTH1 expression on HepG2 tumor in nude mice, and to provide a new strategy for the treatment of HCC. Methods: Human HepG2 cells were cultured and transplanted tumor models were established under the armpit of right forelimb of nude mice. The successful nude mice were randomly divided into two groups: control group (CT) and siRNA transfection group (MTH1). The tumor volume of each group of nude mice was consistent. This experiment was mainly conducted by injecting siRNAs into tumor-bearing nude mice and transfection reagents in vivo. The survival time and living condition of nude mice were observed. The tumor inhibition was evaluated by measuring the volume and weight of solid tumor in nude mice. After the administration, The expression of mth1 and its related genes were detected by qPCR to determine the effect of QPCR on the mRNA expression of each gene. The relative expression of mth1 and related proteins in tumor tissues was detected by Elisa and Western blotting. The effect of siRNA on the protein level of each gene was evaluated by calculating the gray value and comparing the od value. Results: The animal model of HepG2 liver cancer in nude mice was successfully established, and the tumorigenesis rate was 100%. The siRNA transfection group had significant anti-tumor effect and significantly inhibited the growth of tumor cells. Survival rate of nude mice in each group was 100%. Compared with the control group, In the transfected group, the expression of mth1 mRNA and protein decreased significantly in the tumor tissues. The expression of caspase-3 protein was significantly increased in the transfected group. Conclusion: SiRNA-MTH1 can effectively silence the expression of MTH1 gene. Down-regulation of mth1 gene expression by siRNA can inhibit the growth, proliferation and apoptosis of HepG2 tumor cells.

Nude mice, HepG2 CELL, saRNA, MTH1

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