Genetic Polymorphism of Glutathione-S-Transferase Gene (GSTP1) in Type 2 Diabetes Mellitus Patients in Basra Province/Iraq

Author(s): Faizah AW Ahmed, Haneen S Al-bachary

Glutathione S-transferases (GSTs) are enzymes that included, in a wide range of detoxifying reactions by conjugation of glutathione, to electrophilic material. Polymorphisms in the genes that are responsible for GSTs affect, the function of the GSTs. GSTs play an active role in protection of cell against oxidative stress mechanism. Polymorphisms of GSTP1 at codon 105 amino acids forms GSTP1 important site for bind of hydrophobic electrophiles and the substitution of Ile/Val affect substrate specially catalytic activity of the enzyme and may correlate with reach to different diseases in human like diabetes mellitus type2 disease. Correlation between these polymorphisms and changes in the parameters file of diabetic patients has also been found, therefore, the results vary considerably among the studies. The polymerase chain reaction-restriction fragment length polymorphism was used to study GSTP1genetic polymorphism in 60 T2DM patients and 40 healthy individuals. Our results showed that presence of the GSTP1 heterozygous mutant allele Ile/Val was more common in subjects with T2DM than in the control group (35.00% and 17.50.00%, respectively. Among patients there is an association between GSTP1and the risk of T2MD, both genotypes Ile/Val and Val/Val were more prevalent which result in 2.90 and 2.58 respectively risk towards T2DM .According to Hardy–Weinberg principle there was no deviation appears in the distribution of GSTP1 Alleles. GSTP1 genotypes do not have an effect on blood lipids after infection with diabetes mellitus.

GSTP1, Polymorphism, T2DM, Type2 Diabetes Mellitus

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