Identification and Investigation of Phenotypic and Genotypic Characteristics of Flavobacterıum Psychrophılum ın Fry Raınbow Trouts (Oncorhynchus Mykıss) in Some Trout

Author(s): Mikail ÖZCAN, Mustafa SARIEYYÜPOĞLU

In this study, it was aimed to identify and investigate phenotypic and genotypic charecteristics of Flavobacterium psychrophilum in four rainbow trout farms in Keban district of Elazığ province. Two different primers were used to confirm 160 Flavobacterium psychrophilum with PCR after determined according to the phenotype and genotype characteristics. Subsequently, these strains were confirmed as Flavobacterium psychrophilum with DNA. Gene typing was performed with PFGE technique on strains confirmed with PCR. As a result of this, it was decided that strains obtained from four different fish farms wasclose relative with reference Flavobacterium psychrophilum NCIMB 1947T.

Flavobacterium psychrophilum, Rainbow trout, Oncorhynchus mykiss, Polymerase Chain Reaction, PCR, Pulsed Field Gel Electrophoresis, PFGE

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