Prevalence and Morpho-Anatomical Diversity of Arbuscular Mycorrhizal Fungi Spores, from Soybean (Glycine max L.) Rhizosphere in the Agro-Ecological Zone 1 of Cameroon

Author(s): Richard Tobolbaï, Albert Ngakou, Steve Takoukam Toukam

This work investigates on the morpho-anatomical diversity of arbuscular mycorrhiza fungus spores native to soybean rhizosphere in the agro-ecological zone I of Cameroon. Arbuscular mycorrhiza fungi spores have been trapped in pot on composite soils samples taken from three areas in each northern region. Soybean have been used as host plant. After 90 days of growth, the mycorrhizal fungus spores have been extracted and the host plant roots stained. Results analysis revealed that the mycorrhization frequency (F) and intensity (I) are higher at Maroua area (F = 4.6%, I = 3.95%) and lower at Guider zone (F = 1.33% and I = 1.22%). For the specific density, the values vary between 1.54% (Guider zone) and 5.2% (Yagoua zone). Regarding the specific richness, the obtained data fluctuate between 3% (Guider) and 8% (Mokolo). The morpho-anatomical characterization of the spores indicated the presence of 9 different specimens: Septoglomus constrictum, Glomus maculosum, Glomus manihostis, Acaulospora kentinensis, Acaulospore myriocarpa, Rhizophagus intraradices, Ambispora sp, Funneliformis mossea, Diversispora epigae. Among these strains, Septoglomus constrictum is the most abundant specimen while Funneliformis mossea, Ambispora sp and Acaulospora myriocarpa are the rarest. The agronomic performances of these strains can be evaluated for and ecological production of soybeans in the agro-ecological zone I of Cameroon.

Agro-Ecological Zone 1, Specfic Density, Specific Richness, Mycorrhization Frequency, Myorrhization Intensity

1. Anissa Lounes-Hadj Sahraoui, 2013. La Mycorhize a arbuscules : quels bénéfices pour I'homme et son environnement dans un contexte de développement durable ? Rev. Sci. Technol., Synthese 26: 06 -19.
2. Genot V., Colinet G., Bock L., 2007. Fertilité du sol agricole et forestier en région Wallone. Dossier scientifique, rapport analytique 2006-2007 sur l’état de l’environnement Wallonne. Faculté universitaire agronomiques de Gembloux, 13p.
3. Głodowska M., Wozniak M., 2019. Changes in Soil Microbial Activity and Community Composition as a Result of Selected Agricultural Practices. Agricultural Sciences, 10 : 330-351.
4. Gnamkoulamba A., Tounou A. K., Tchabi A., Agboka K., Adjévi A. K. M., Batawila K., 2018. Prévalence et diversité des spores des champignons mycorhiziens arbusculaires en culture de riz sous les différents systèmes de culture de riz dans cinq zones agro-écologiques au Togo. Journal of Applied Biosciences, 126: 12647-12664.
5. Helgason T., Daniell T. J., Husban R., Fitter A. H., Young J. P. Y., 1998. Ploughing up the wood-wide web ? Nature, 394 : 431.
6. Kaonongbua W., Morton J. B. & Bever J. D., 2010. Taxonomic revision transferring species in Kuklospora to Acaulospora (Glomeromycota) and a description of Acaulospora colliculosa sp from field collected spores. Mycologia, 102, 1497-1509.
7. Maurer C., Rüdy M., Chervet A., Sturny W. G., Flisch R. & Oehl F., 2014. Diversité des champignons mycorhiziens Arbusculaires sous semis direct et sous labour. Recherche Agronomique Suisse, 5 (10) : 398-405.
8. Miller D. D. & Walker C: 1986. Glomus maculosum sp. nov. (Endogonaceae): an endomycorrhizal fungus. Mycotaxon, 26 : 217-227.
9. Nadjilom Y., Toukam S. T., Tobolbaï R. & Ngakou1 A., 2019. Morphological and structural characterization of rhizospheric endomycorrhiza communities associated with rice grown in the sahelian zone (Chad). International Journal of Plant & Soil Science 31(5): 1-14.
10. Ngonkeu M. E. L., Nwaga D., Adamou S., Fokom R., Tchameni N. S., Onguene N. A., Nana W. L., Chaintreuil C., The C., Amougou A., Moulin L., Prin Y., Lebrun M., Dreyfus B. Diversite des champignons mycorhiziens arbusculaires du cameroun. IRD Editions. Institut de Recherche pour le Développement Marseille, 2013.
11. Ouallal I., Abbas Y., Ech-cheddadi S., Ouajdi M., Ouhadach M., Yacoubi H. E., Kerdouh B., Goumi Y. E., Rochdi A., 2018. Diversité des champignons endomycorhiziens de l’arganier et potentiel mycorhizogène des sols rhizosphériques des arganeraies du Sud-Ouest marocain, Bois et Forêts des Tropiques, 338 : 73-86.
12. Schenck NC. & Smith GS: 1982. Additional new and unreported species of mycorrhizal fungi (Endogonaceae) from Florida. Mycologia, 74, 77-92.
13. Schenck N. C. & Smith G. S: 1982. Additional new and unreported species of mycorrhizal fungi (Endogonaceae) from Florida. Mycologia, 74 : 77-92.
14. Schenck NC, Spain JL, Sieverding E. & Howeler RH: 1984. Several new and unreported vesicular-arbuscular mycorrhizal fungi (Endogonaceae) from Colombia. Mycologia, 76, 685-699.
15. Sghir F, Chliyeh M, Kachkouch W, Khouader M, Touhami A, Benkirane R. & Douira
16. A: 2013. Mycorrhizal status of Olea europaea spp. oleaster in Morocco. Journal of Applied Biosciences, 61, 4478 - 4489.*
17. Schenck NC, Spain JL, Sieverding E. & Howeler RH: 1984. Several new and unreported vesicular-arbuscular mycorrhizal fungi (Endogonaceae) from Colombia. Mycologia, 76 : 685-699.
18. Schenck N. C. and Peres Y., 1990. Manual for the identification of VA mycorrhizal fungi, 3rded. Synergistic Publ., Gainesville, FL.
19. Shannon CE. 1948. A mathematical theory of communications. Bell Systems Technical
20. Journal, 27, 379-423.
21. Tobolbai R., Adamou S., Ngakou A., 2018. Morphological and structural diversities of indigenous endomycorrhiza communities associated to maize [Zea mays (L.)] in Northern Cameroonian soils. Journal of Animal & Plant Sciences, 38, (1): 6057-6073.
22. Trappe JM: 1977. Three new Endogonaceae: Glomus constrictus, Sclerocystis clavispora, and Acaulospora scrobiculata. Mycotaxon, 6, 359-366.
23. Walker C., 1979. Complexipes moniliformis: A new genus and species tentatively, placed in the Endogonaceae. Mycotaxon, 12 : 99-104.
24. Yolande Dalpé, 2005. Les mycorhizes : un outil de protection des plantes mais non une Panacée. Phytoprotection 86 : 53-59.
25. Zougari-Elwedi B., Mustapha, Labibdi S., Dalpé Y. & Lounès-Haj S. A., 2012. Evaluation de la mycorhizaion du palmier dattier (Phoenix dactylifera L.) dans la région du Djérid (1), Sanaa Revue de l’I.N.A.T. Vol 28 n° 1 – juin 2012.